LOCUS       40924_33662        4411 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNV19 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa
            J.
  TITLE     Construction of a pair of practical Nocardia-Escherichia coli 
            shuttle vectors
  JOURNAL   Jpn. J. Infect. Dis. 60 (1), 45-47 (2007)
  PUBMED    17314425
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Ishikawa J, Chiba K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2006) Contact:Jun Ishikawa National Institute of 
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1 
            Toyama, Shinjuku, Tokyo 162-8640, Japan URL 
            :http://nocardia.nih.go.jp/
REFERENCE   3  (bases 1 to 4411)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Jpn. J. 
            Infect. Dis."; date: "2007"; volume: "60"; issue: "1"; pages: 
            "45-47"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2006) Contact:Jun Ishikawa National Institute of Infectious 
            Diseases, Department of Bioactive Molecules; 1-23-1 Toyama, 
            Shinjuku, Tokyo 162-8640, Japan URL :http://nocardia.nih.go.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Dec 18, 2007 this sequence version replaced AB267086.1.
FEATURES             Location/Qualifiers
     source          1..4411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    169..225
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(238..254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(262..278)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(286..316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(331..352)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(640..1228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1515..2306)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2662..4411
                     /label=derived from pAL5000
                     /note="derived from pAL5000"
     CDS             complement(2746..3093)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein RepB"
                     /label=repB
                     /protein_id="BAF02360.2"
                     /translation="MSDGYSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQER
                     SEWLAEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQK
                     AHNEADNPPLF"
     gene            complement(2746..3093)
                     /gene="repB"
                     /label=repB
     CDS             complement(3090..4016)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein RepA"
                     /label=repA
                     /protein_id="BAF02361.1"
                     /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP
                     TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR
                     RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN
                     MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC
                     HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS
                     RKGAAARTAASTVARRAKSASAMEALL"
     gene            complement(3090..4016)
                     /gene="repA"
                     /label=repA
ORIGIN
        1 agcttatcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
       61 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      121 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc
      181 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc
      241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
      841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1081 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1321 cttcacctag atccttttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     1381 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     1441 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     1501 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     1561 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     1621 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     1681 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     1741 ccatgggtca cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt
     1801 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     1861 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     1921 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     1981 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2041 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2101 cacgatagcc gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg
     2161 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     2221 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     2281 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     2341 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     2401 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     2461 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     2521 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     2581 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     2641 tgcttgcggc agcgtgaagc tagcgtctac caggactttt acctgtccga cccgttgcaa
     2701 cggaaccccc cacggaaccc ccgcgacacc cgctccccaa ttgcgttaga acagcggtgg
     2761 attgtcggct tcgttgtggg ccttttgagc cgcttcctgt tctgccgcac gctctttcct
     2821 cgcccgatag ccgagtcgct taacggtgtc cagatgcagc ccgaaatgtt tggccgtttg
     2881 cggccaagag tggccctcgt cgtcgtgata ggcgcggatg cgttcgcggc gtgcagcctg
     2941 ctcggcgagc cactcgctgc gttcctgcgc cacgagccgg acgacgtggc gttcggatag
     3001 tccggtgatt cgagcgcctt cggcggcggt cacgcgccgc tttttgcgga cagtcggctg
     3061 ccggttgtag ccgtcgctgt agccgtcgct catagcaatg cctccatggc tgacgcggac
     3121 tttgcgcgcc gcgcaactgt gctcgccgcc gtgcgcgctg ctgcgccctt ccgcgagatg
     3181 gccgactggc gcgcactgag tgtggcctcg tagaccacga tcccgtccgc ccaaatgcgc
     3241 gacttggttg tgatccaacg ccaaatgctg ttggcgatgg cgcggacctc gctgtccggt
     3301 agcggtccgg gacacacgtc gttgcacggg aactcggcgt ttcgcgcgtg gcactcggca
     3361 tagatcgcgc ggccgagtcc gtccacgttc cgggtcggca ggtagatccg catgagggcg
     3421 ggacgatagg cccacaacct gacggaatcg aacagtgcgc aattccgccc tagcggcgtc
     3481 ggagccgctt tgtacgtggt ctgctgacgc cagcgcggcg gtggcatgtt cgcgccgagc
     3541 tcggcctcga tgtggctgag tgtgtagaga tctgagtgga gccattccgt ttcccaggcg
     3601 atgtggccgg ggtttttggt catgaggcct gagtaactgc ggtcgccgtc cacggcgcgc
     3661 cgaaggcctt cggcgcacgc cgccatgtat gcgagcggct tacgccgcgc gtattcggtg
     3721 cgtggaacag gggcgttgag tgcccacact gcgtgtgcgt ggccgttggc gcgattgccc
     3781 acgatcgcgt tgggcagcgg atgggacccc cgggcgctga gcgctcggag cgctgcgtct
     3841 ggatggtcta cgtccacgac cagcaggttt gccagcgctg ttgggttcgc ctcgatgtac
     3901 cggcggccta gggccgacgc gcggctttgg cggtagatcc cctcgagcag atcgtcgctt
     3961 gccagcggcc agtacggcag ccagagctgc tcaaattcgt cggcgacgtg gctcacgctt
     4021 ggtagtagac cacgattaat caccggtgta tggtccgaca cgagctccaa gtcagatatt
     4081 tcgctgaggg gccaccccac aactgcacac tcccccgctc tcccgtcgag ccctggtggt
     4141 ggaacaccag cgacagccga gcacccccaa ccacctgtac caaccaggag gaacacatgc
     4201 gtcgtttcga ggacgtttcc gggccgctga gagccgctgt ggcggccgta cacgccgcct
     4261 tagacccgtt agaccccctg ccgcctgaat gcgcgggtac gagccacaca gcgcccgaac
     4321 ttacggagct ggtgggctca cctggcttta tggcgtacga atcggctgtg tgcgacctgt
     4381 tgggcgaggt gaggtacgcg ctactcacgc t
//
